Molecular Reagents： Isothermal Amplification

Isothermal amplification (including HAD, MCA, and LAMP) is a DNA amplification technique that has played an increasingly important role in point-of-care (POC) diagnostics. It offers several advantages over qPCR and other amplification techniques in terms of sample-to-answer time, sensitivity, specificity, cost, robustness, and accessibility, making it ideal for field-deployable diagnostics in resource-limited regions. Isothermal amplification assays have been used for numerous applications including the detection of pathogens such as salmonella and malaria, GM crop contamination, and forensics to specifically detect human DNA.